Part:BBa_K208000:Design

GFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 4
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 523
  Illegal XhoI site found at 424
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

In plasmid pSB1AK3
Silver fusion compatible

Source

This is a cycle 3 mutant developed by Crameri et al. (1996). It was isolated using PCR. The designed primers had the Silver fusion prefix and suffix as non-attaching overhangs. Following PCR with these primers, the product was cut with BioBrick restriction enzymes and ligated into the previously cut BioBrick vector pSB1AK3.

References

1. Chalfie M, Tu Y, Euskirchen G, Ward W, and Prasher D. (1994) Green fluorescent protein as a marker for gene expression. Science 263:802-805
2. Crameri A, Whitehorn E, Tate E, Stemmer W. (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotech 14:315-19
3. Lavallie ER, McCoy JM (1995) Gene fusion expression systems in Escherichia coli.Current Biology Ltd 501-506
4. NCBI. http://www.ncbi.nlm.nih.gov/nuccore/1490531?from=1342&to=2061&report=gbwithparts#sequence_1490531